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Updated: Mar 18, 2026

Reconstruct Human Retinoblastoma In Vitro
Published on: October 11, 2022
Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
Heema Hewitson1, Victoria Wood1, Neli Kadeva1
1Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
Abstract:
The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

