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Related Experiment Videos

Adipsin and complement factor D activity: an immune-related defect in obesity.

B S Rosen1, K S Cook, J Yaglom

  • 1Dana-Farber Cancer Institute, Boston, MA.

Science (New York, N.Y.)
|June 23, 1989
PubMed
Summary

Adipsin, a protein deficient in obesity models, functions like complement factor D. This suggests adipsin and the complement system are unexpectedly involved in regulating energy balance.

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Area of Science:

  • Biochemistry
  • Immunology
  • Metabolism

Background:

  • Adipsin, a serine protease from adipocytes, is deficient in obesity models.
  • Defective gene expression is linked to obesity.
  • The function of adipsin remains largely unelucidated.

Purpose of the Study:

  • To purify and characterize recombinant mouse adipsin.
  • To elucidate the biochemical and enzymatic properties of adipsin.
  • To investigate the role of adipsin in complement activation and energy balance.

Main Methods:

  • Purification of recombinant mouse adipsin.
  • Biochemical and enzymatic assays to determine adipsin activity.
  • Assessment of complement alternative pathway activation and red blood cell lysis.

Related Experiment Videos

  • Comparison of adipsin activity in various obesity models.

    • Main Results:

    • Activated adipsin exhibits complement factor D-like activity, cleaving complement factor B.
    • Adipsin activates the alternative pathway of complement, leading to red blood cell lysis.
    • Significantly decreased complement factor D activity (58-80%) was observed in multiple obesity models (ob/ob, db/db, MSG-injected mice, fa/fa rats).

    Conclusions:

    • Adipsin functions analogously to complement factor D.
    • Adipsin activates the complement alternative pathway.
    • Adipsin and the complement alternative pathway may play a crucial role in regulating systemic energy balance.