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Imaging Bioorthogonal Groups in Their Ultrastructural Context with Electron Microscopy.

Daphne M van Elsland1, Sander I van Kasteren2

  • 1Leiden Institute of Chemistry/Institute of Chemical Immunology, Bioorganic Synthesis, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.

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Three-dimensional electron microscopy can now image bioorthogonal groups within cells. This technique provides crucial structural insights into these molecules in their native cellular environment.

Keywords:
electron microscopyfluorescenceimagingphotochemistrysinglet oxygen

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Area of Science:

  • Biochemistry
  • Microscopy
  • Cell Biology

Background:

  • Bioorthogonal chemistry enables studying biological processes.
  • Visualizing bioorthogonal groups in situ is challenging.
  • Advanced imaging techniques are needed for cellular context.

Purpose of the Study:

  • To discuss a recent paper on imaging bioorthogonal groups.
  • To evaluate three-dimensional electron microscopy for this purpose.
  • To demonstrate its suitability for cellular structural information.

Main Methods:

  • Three-dimensional electron microscopy.
  • Imaging of bioorthogonal groups.
  • Analysis of cellular context.

Main Results:

  • Electron microscopy successfully imaged bioorthogonal groups.
  • Structural information was obtained.
  • Imaging was performed within the cellular context.

Conclusions:

  • Three-dimensional electron microscopy is a viable technique for imaging bioorthogonal groups.
  • It provides valuable structural insights.
  • The method is suitable for studying these groups in their native cellular environment.