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Updated: Mar 18, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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miR-10b promotes invasion by targeting HOXD10 in colorectal cancer

Yunfeng Wang1, Zhen Li1, Xuhong Zhao2

  • 1Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.

Oncology Letters
|June 28, 2016
PubMed

Insights

MicroRNA-10b (miR-10b) promotes colorectal cancer (CRC) invasion by targeting HOXD10, leading to increased RhoC expression. This pathway contributes to tumor metastasis in CRC patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Homeobox D10 (HOXD10) is a target of microRNA-10b (miR-10b), inhibiting cell migration and invasion.
  • Ras homolog family member C (RhoC) promotes tumor metastasis in various cancers.
  • The role of miR-10b in colorectal cancer (CRC) metastasis is not fully understood.

Purpose of the Study:

  • To investigate if miR-10b promotes CRC invasion by targeting HOXD10.
  • To explore the association between miR-10b and HOXD10 expression in CRC patients.

Main Methods:

  • Analysis of miR-10b and HOXD10 expression in CRC specimens.
  • Correlation analysis between miR-10b, HOXD10, and RhoC expression.
  • Assessment of HOXD10 protein levels in relation to lymph node metastasis.

Main Results:

  • miR-10b levels were elevated in CRC specimens and correlated with advanced stage and lymph node metastasis.
  • HOXD10 is a direct target of miR-10b.
  • Increased RhoC and decreased HOXD10 expression correlated with elevated miR-10b.
  • HOXD10 protein was downregulated in lymph node metastasis-positive tissues.

Conclusions:

  • miR-10b targets HOXD10 in colorectal cancer.
  • miR-10b may promote CRC invasion and migration by upregulating RhoC via HOXD10 downregulation.
  • Findings elucidate a molecular mechanism for miR-10b in CRC metastasis.
Keywords:
colorectal cancerhomeobox D10microRNA-10bmigration

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