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Updated: Mar 18, 2026
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
Development and characterization of SSR markers for Aster savatieri (Asteraceae)
Naoko Ishikawa1, Shota Sakaguchi2, Motomi Ito1
1Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo 153-0041, Japan.
Premise Of The Study:
Simple sequence repeat (SSR) markers were developed for Aster savatieri (Asteraceae) and the serpentine variety A. savatieri var. pygmaeus to re-evaluate their taxonomic status.
Methods And Results:
Using RNA-Seq data, 22 expressed sequence tag (EST)-SSR markers were developed. Polymorphisms were assessed in A. savatieri and in A. savatieri var. pygmaeus. The average number of alleles ranged from four to 15, and expected heterozygosity ranged from 0.417 to 0.870. Transferability was examined in six representative species of Japanese Aster and in Solidago virgaurea subsp. asiatica var. asiatica, a member of the tribe Astereae (Asteraceae); most of the loci were transferable to these examined species.
Conclusions:
These markers will be useful for genetic studies of variation in A. savatieri and other Aster species that occur in Japan.
