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Updated: Mar 18, 2026

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
Published on: August 14, 2019
Replication-Competent Influenza A Viruses Expressing Reporter Genes
Michael Breen1, Aitor Nogales2, Steven F Baker3
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA. michael.breen24@gmail.com.
Replication-competent Influenza A viruses (IAV) engineered to express reporter proteins simplify identifying infected cells. These recombinant IAV offer a powerful tool for studying IAV in vitro and in vivo, advancing research on influenza.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Influenza A viruses (IAV) cause significant seasonal epidemics and occasional pandemics.
- Current methods for detecting IAV-infected cells are laborious and time-consuming.
- A need exists for more efficient tools to study IAV replication and infection dynamics.
Purpose of the Study:
- To develop and discuss the applications of recombinant replication-competent IAV expressing reporter genes.
- To provide a simplified method for identifying virus-infected cells in various research settings.
- To highlight the utility of these reporter viruses in advancing influenza A virus research.
Main Methods:
- Development of replication-competent IAV engineered to harbor fluorescent or bioluminescent reporter genes.
- Integration of reporter genes at diverse locations within the viral genome.
- Utilizing these recombinant viruses for both in vitro and in vivo experimental models.
Main Results:
- Successfully created replication-competent IAV expressing traceable reporter proteins.
- Demonstrated the utility of these reporter viruses in various applications.
- Enabled simplified identification of infected cells, reducing experimental complexity.
Conclusions:
- Reporter-expressing, replication-competent IAV are a powerful tool for studying influenza.
- These recombinant viruses facilitate diverse research applications, including drug screening and vaccine development.
- The developed viruses significantly enhance the efficiency of in vitro and in vivo IAV research.
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