Related Experiment Video

Updated: Mar 18, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

1.1K

Ras-association domain family 10 acts as a novel tumor suppressor through modulating MMP2 in hepatocarcinoma

W Liu1,2, J Wang3, L Wang1,2

  • 1Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Oncogenesis
|June 28, 2016
PubMed

Insights

Ras-Association Domain Family 10 (RASSF10) acts as a tumor suppressor in hepatocellular carcinoma (HCC). Silencing of RASSF10 promotes HCC cell viability, growth, and metastasis, indicating its crucial role in liver cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ras-Association Domain Family 10 (RASSF10) is a recently identified member of the RASSF family.
  • Its specific functions in human cancers, particularly hepatocellular carcinoma (HCC), are not well understood.

Purpose of the Study:

  • To investigate the biological functions and molecular mechanisms of RASSF10 in hepatocellular carcinoma (HCC).
  • To determine if RASSF10 acts as a tumor suppressor in HCC development and progression.

Main Methods:

  • Analysis of RASSF10 expression in HCC cell lines and primary tumors.
  • Assessment of promoter methylation as a mechanism for RASSF10 inactivation.
  • In vitro and in vivo experiments using transgenic RASSF10 expression in HCC cell lines.
  • Cell cycle analysis and investigation of downstream effectors, including MMP2, Focal Adhesion Kinase, and p38 MAPK.

Main Results:

  • RASSF10 is frequently silenced or downregulated in HCC cell lines and tumors, often due to promoter methylation.
  • Restored RASSF10 expression suppressed HCC cell viability, colony formation, and tumor growth in vivo.
  • RASSF10 induced G1 cell cycle arrest and inhibited HCC cell migration and invasion.
  • RASSF10's tumor-suppressive effects were partially mediated by regulating MMP2, Focal Adhesion Kinase, and p38 MAPK signaling.

Conclusions:

  • RASSF10 functions as a tumor suppressor in hepatocellular carcinoma.
  • RASSF10 inactivation through promoter methylation contributes to HCC progression.
  • RASSF10 represents a potential therapeutic target for HCC.

More Related Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.3K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.7K

Related Concept Videos

The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.2K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

2.0K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K