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    Area of Science:

    • Sports Science
    • Biochemistry
    • Analytical Chemistry

    Background:

    • The World Anti-Doping Agency (WADA) is expanding its focus beyond small molecules to include larger endogenous molecules for doping detection.
    • Implementation of an endocrinological module within the Athlete Biological Passport (ABP) is a key WADA initiative.

    Purpose of the Study:

    • To discuss the detection of growth hormone (GH) and endogenous anabolic androgenic steroids (EAASs) for doping control.
    • To outline current research trends and state-of-the-art detection methods for these substances.
    • To introduce microRNAs (miRNAs) as novel biomarkers for anti-doping efforts.

    Main Methods:

    • Review of historical context and current analytical techniques used in accredited anti-doping laboratories.
    • Exploration of advanced methodologies for detecting hormones (GH, EAASs) and microRNAs (miRNAs).

    Main Results:

    • Established methods for detecting GH and EAASs are in use.
    • Emerging research focuses on refining these detection techniques and exploring new biomarkers.
    • MicroRNAs (miRNAs) show promise as a new class of doping biomarkers.

    Conclusions:

    • WADA's strategic shift towards detecting larger endogenous molecules like hormones is advancing anti-doping capabilities.
    • State-of-the-art methods and ongoing research are crucial for effective detection of GH, EAASs, and novel biomarkers like miRNAs.
    • The integration of endocrinological analysis and new biomarkers will strengthen the Athlete Biological Passport (ABP).