New ent-kaurane diterpenoid dimer from Pulicaria inuloides.

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Pharmacognosy

Background:

• Pulicaria inuloides is a medicinal plant with potential therapeutic properties.
• Diterpenoid dimers are complex natural products with diverse biological activities.

Purpose of the Study:

• To isolate and characterize chemical constituents from the aerial parts of Pulicaria inuloides.
• To evaluate the isolated compounds for antimicrobial, cytotoxic, and antioxidant activities.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation using 1D and 2D NMR spectroscopy and ESI-MS.
• Biological activity evaluation including antimicrobial, cytotoxic (HepG2 cell line), and antioxidant assays.

Main Results:

• A novel ent-kaurane diterpenoid dimer (1) and six known compounds (2-7) were isolated.
• Compounds 2-5 were reported for the first time in the genus Pulicaria.
• The isolated compounds exhibited varying degrees of antimicrobial, cytotoxic, and antioxidant activities.

Conclusions:

• The study identified new and known compounds from Pulicaria inuloides.
• The isolated compounds possess potential for further development as therapeutic agents.
• This research contributes to the understanding of the phytochemical profile and bioactivity of Pulicaria inuloides.