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Related Experiment Videos

Structure and function of the androgen receptor.

A O Brinkmann1, P Klaasen, G G Kuiper

  • 1Department of Biochemistry II, Erasmus University, Rotterdam, The Netherlands.

Urological Research
|January 1, 1989
PubMed
Summary

The androgen receptor, a key protein in gene regulation, has distinct functional domains. A fragment containing DNA and steroid binding sites showed enhanced DNA binding activity.

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Area of Science:

  • Molecular biology
  • Endocrinology
  • Genetics

Background:

  • The androgen receptor (AR) is a crucial protein involved in regulating gene expression.
  • Understanding the AR's structure and function is vital for comprehending androgen-mediated biological processes.

Purpose of the Study:

  • To characterize the structural and functional domains of the androgen receptor.
  • To investigate the DNA binding activity of different androgen receptor fragments.

Main Methods:

  • Protein molecular mass determination (100-110 kDa).
  • Identification and size analysis of DNA-binding (approx. 10 kDa) and steroid-binding (30 kDa) domains.
  • Isolation of human androgen receptor cDNA.
  • Comparative sequence homology analysis with progesterone and glucocorticoid receptors.

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Main Results:

  • A 40 kDa fragment encompassing both DNA and steroid binding domains exhibited higher DNA binding activity than the intact receptor.
  • Human androgen receptor cDNA revealed an open reading frame of 2,277 bp.
  • Significant homology was observed between androgen, progesterone, and glucocorticoid receptors, particularly in the DNA-binding domain (approx. 80%).

Conclusions:

  • The androgen receptor is a monomeric protein with distinct functional domains.
  • The androgen receptor belongs to the superfamily of ligand-responsive transcriptional regulators.
  • Structural organization of the androgen receptor supports its role in specialized transcriptional regulation.