Related Experiment Video
Updated: Mar 18, 2026
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
Comprehensive investigation of aberrant microRNA profiling in bladder cancer tissues
Yanping Wei1, Rongquan He1, Yuzhuang Wu2
1Center for Genomic and Personalized Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, 530021, China.
Abstract:
There has been accumulative evidence that microRNAs (miRNAs) play essential roles in the tumorigenesis and progression of bladder cancer. However, individual studies and small sample size caused discrepant outcomes. Thus, the current study focused on a comprehensive profiling of all differentially expressed miRNAs in a total of 519 bladder cancer tissue samples, based on miRNA microarray data. Altogether, 11 prioritized miRNAs stated by 21 published microarray datasets, including five down-regulated (miR-133a-3p, miR-1-3p, miR-99a-5p, miR-490-5p, and miR-133b) and six up-regulated candidate miRNAs (miR-182-5p, miR-935, miR-518e-3p, miR-573, miR-100-3p, and miR-3171) were analyzed with vote-counting strategy and a Robust Rank Aggregation method. Subsequently, miRNA in silico target prediction and potential pathway enrichment analysis were performed to investigate the prospective molecular mechanism of miRNAs in the tumorigenesis of bladder cancer. We found that most of the relative pathways of the aberrantly expressed miRNAs found in the current study were closely correlated with different biological processes, cellular components, molecular functions, cancer pathogeneses, and some cell signalings, such as Wnt signaling, insulin/IGF, PI3 kinase, and FGF signaling pathways. Hence, a comprehensive overview on the miRNA expression pattern in bladder cancer tissues was gained by the current study. These miRNAs might be involved in the tumorigenesis and deterioration of bladder cancer.
Insights
MicroRNAs (miRNAs) are crucial in bladder cancer development. This study identified 11 key miRNAs and their associated signaling pathways, offering insights into bladder cancer progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are increasingly recognized for their roles in bladder cancer tumorigenesis and progression.
- Previous studies have yielded inconsistent results due to small sample sizes.
- A comprehensive analysis is needed to clarify the role of miRNAs in bladder cancer.
Purpose of the Study:
- To conduct a comprehensive profiling of differentially expressed miRNAs in bladder cancer.
- To identify key miRNAs involved in bladder cancer development and progression.
- To investigate the molecular mechanisms and signaling pathways influenced by these miRNAs.
Main Methods:
- Analysis of miRNA microarray data from 519 bladder cancer tissue samples.
- Utilized vote-counting and Robust Rank Aggregation (RRA) methods to prioritize 11 differentially expressed miRNAs from 21 published datasets.
- Performed in silico target prediction and pathway enrichment analysis.
Main Results:
- Identified 11 prioritized miRNAs, including five down-regulated (e.g., miR-133a-3p) and six up-regulated (e.g., miR-182-5p).
- Found that aberrant miRNA expression correlates with various biological processes, cellular components, molecular functions, and cancer pathogenesis.
- Associated pathways include Wnt signaling, insulin/IGF, PI3 kinase, and FGF signaling.
Conclusions:
- This study provides a comprehensive overview of miRNA expression patterns in bladder cancer.
- The identified miRNAs are potentially involved in the initiation and progression of bladder cancer.
- These findings may contribute to understanding bladder cancer etiology and developing targeted therapies.
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs