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Mutant allele of rna14 in fission yeast affects pre-mRNA splicing

Sudhanshu Yadav1, Amit Sonkar, Nafees Ahamad

  • 1Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226 031, India.shakil_ahmed@cdri.res.in.

Journal of Genetics
|June 29, 2016
PubMed

Insights

The Rna14 protein in fission yeast is crucial for precursor messenger RNA (pre-mRNA) splicing, impacting Chk1 protein levels. This study reveals Rna14

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Precursor mRNA (pre-mRNA) maturation involves spliceosome and 3'-end processing complexes.
  • Rna14 protein in budding yeast is known to be involved in mRNA cleavage and polyadenylation.
  • The role of Rna14 in pre-mRNA splicing remains uninvestigated.

Purpose of the Study:

  • To investigate the function of Rna14 in pre-mRNA processing in fission yeast.
  • To determine if Rna14 plays a role in pre-mRNA splicing.

Main Methods:

  • Isolation and characterization of a mutant rna14 allele in Schizosaccharomyces pombe.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to assess gene splicing.
  • Genetic interaction studies with prp1 and physical interaction studies with Prp28.

Main Results:

  • A mutant rna14 allele caused reduced Chk1 protein levels at nonpermissive temperatures due to posttranscriptional processing defects.
  • RT-PCR analysis showed defective splicing of the chk1(+) transcript and other genes.
  • Rna14 demonstrated genetic interaction with prp1 and physical interaction with Prp28.

Conclusions:

  • Rna14 is involved in pre-mRNA splicing in fission yeast.
  • Rna14 may be a component of a larger protein complex essential for pre-mRNA maturation.

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