Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum to "Dysregulation of the Cant1/beta-Catenin/TCF4-CHSY1 Axis Underpins Impaired ECM Biosynthesis in Skeletal Disorders".

Research (Washington, D.C.)·2026
Same author

Effect of Short-Term Grape Powder Supplementation in Patients with Crohn's Disease: A Pilot Study.

Nutrients·2026
Same author

A Randomized Controlled Trial Comparing Soy-Pea Protein to Animal Protein in Adults with Crohn's Disease.

medRxiv : the preprint server for health sciences·2026
Same author

Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in <i>Pseudomonas aeruginosa</i>.

eLife·2026
Same author

Dysregulation of the Cant1/β-Catenin/TCF4-CHSY1 Axis Underpins Impaired ECM Biosynthesis in Skeletal Disorders.

Research (Washington, D.C.)·2026
Same author

Inflammatory bowel disease patients believe cannabis and cannabidiol oil relieve symptoms.

Academia medicine·2025

Related Experiment Video

Updated: Mar 18, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

10.5K

False-Positive Clostridium difficile in Negative-Control Reactions Peak and Then Decrease with Repetitive

Alexander Rodriguez-Palacios1, Henry R Stämpfli2, Yung-Fu Chang3

  • 1Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14580, USA; Division of Gastroenterology and Liver Disease, Case Western Reserve University, Cleveland, OH 44106, USA.

International Scholarly Research Notices
|June 29, 2016
PubMed
Summary
This summary is machine-generated.

Repeatedly refrigerating enzyme-linked immunosorbent assay (ELISA) negative controls can cause false-positive results, potentially leading to misdiagnoses. Using multiple negative controls is crucial for accurate Clostridium difficile testing.

More Related Videos

Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

1.4K
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

9.3K

Related Experiment Videos

Last Updated: Mar 18, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

10.5K
Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

1.4K
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

9.3K

Area of Science:

  • Clinical diagnostics
  • Immunology
  • Microbiology

Background:

  • Enzyme-linked immunosorbent assays (ELISA) are widely used for diagnosing infections like Clostridium difficile.
  • False-positive results in diagnostic tests can lead to incorrect patient management and unnecessary treatments.
  • Preanalytical variables, such as temperature fluctuations, can significantly impact immunoassay performance.

Purpose of the Study:

  • To investigate the impact of repeated refrigeration-to-room temperature cycles on the reliability of negative-control reagents in ELISA testing for Clostridium difficile.
  • To determine the optimal number of negative controls needed to ensure accurate diagnostic results under such conditions.

Main Methods:

  • Experiments were conducted using 96-well ELISA products.
  • Negative-control reagents were subjected to multiple cycles of refrigeration and room temperature exposure.
  • The frequency and intensity of false-positive immunoassay reactions were quantified and analyzed statistically.

Main Results:

  • A significant peak in false-positive immunoassay reactions was observed after 5 refrigeration-to-room temperature cycles (P < 0.001).
  • False-positive reactions decreased with further cycles of refrigeration.
  • The study highlights the susceptibility of negative-control reagents to temperature-related degradation.

Conclusions:

  • Repetitive temperature cycling of negative controls can induce aberrant false-positive results in Clostridium difficile ELISA testing.
  • Relying on single negative controls per ELISA run may be insufficient to detect preanalytical false-positives when reagents undergo repeated refrigeration.
  • Implementing multiple negative controls per run is recommended to enhance the accuracy and reliability of ELISA diagnostics susceptible to preanalytical variability.