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Related Experiment Video

Updated: Mar 18, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Droplet barcoding for massively parallel single-molecule deep sequencing.

Freeman Lan^1,2, John R Haliburton^1,3, Aaron Yuan^1,4

¹Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, California 94158, USA.

Nature Communications

|June 30, 2016

Summary

This summary is machine-generated.

This study introduces a novel droplet microfluidics method to achieve long and accurate DNA sequencing using short-read technologies. This technique enhances mutation detection and haplotype linkage in complex biological samples.

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Last Updated: Mar 18, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Accurate long DNA molecule sequencing is crucial for biological research.
Current short-read sequencing technologies have limitations in read length and accuracy.
There is a need for improved methods to sequence long DNA fragments comprehensively.

Purpose of the Study:

To develop a method for obtaining long and accurate DNA reads by leveraging short-read sequencing.
To overcome the limitations of existing DNA sequencing technologies.
To enable the identification of rare mutations and direct haplotype linkage.

Main Methods:

Utilizing droplet microfluidics to isolate, amplify, fragment, and barcode single DNA molecules.
Sequencing full-length DNA molecules within aqueous picolitre droplets.
Employing multi-fold coverage with short-read sequencing for enhanced accuracy.

Main Results:

Demonstrated accurate sequencing of DNA molecules up to 10 kilobases (kb).
Enabled identification of rare mutations previously undetectable by conventional sequencing methods.
Facilitated direct linkage of mutations into haplotypes.

Conclusions:

The developed barcoding methodology offers a powerful tool for sequencing heterogeneous populations, including viruses.
This approach significantly improves the accuracy and read length achievable with short-read sequencing.
The method has broad applications in genomics and molecular diagnostics.