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Related Experiment Video

Updated: Mar 18, 2026

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Published on: December 13, 2024

Multiple genetically engineered humanized microenvironments in a single mouse.

Jungwoo Lee¹, Dirk Heckl², Biju Parekkadan³

¹Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital & Harvard Medical School and Shriners Hospital for Children, Boston, MA USA ; Department of Chemical Engineering, Institute for Applied Life Sciences, University of Massachusetts, Amherst, MA USA.

Biomaterials Research

|June 30, 2016

Summary

This summary is machine-generated.

This study developed a novel mouse model using biomaterial implants to create localized human cytokine microenvironments. This platform enables the study of human stem cell engraftment and differentiation in vivo.

Keywords:

Bone marrow stromal cells Chimeric mouse model Genetically engineered stroma Human hematopoietic stem and progenitor cells Implantable microenvironments Scaffolds

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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Area of Science:

Biomedical Engineering
Stem Cell Biology
Immunology

Background:

Immunodeficient mouse models are crucial for human stem cell research.
Previous models faced limitations in studying localized human cell behavior.
This study aimed to engineer specific microenvironments for human cell studies.

Purpose of the Study:

To create a novel in vivo system for studying human CD34+ hematopoietic stem/progenitor cell (HSPC) engraftment and differentiation.
To engineer localized microenvironments within a single mouse using biomaterial implants and genetically engineered stromal cells.
To investigate the effects of specific human cytokines on HSPC behavior.

Main Methods:

Utilized subdermal biomaterial implants seeded with genetically engineered stromal cells.
Engineered stromal cells to overexpress specific human cytokines: Vascular Endothelial Growth Factor A (hVEGFa), Stromal Derived Factor 1 Alpha (hSDF1a), or Tumor Necrosis Factor Alpha (hTNFa).
Controlled stromal cell growth to minimize systemic cytokine exposure, creating autonomous local sites.

Main Results:

Successfully created localized microenvironments expressing specific human cytokines within a single mouse.
Demonstrated minimized systemic exposure of locally overexpressed cytokines.
Validated that engineered factors had bioactive effects on human CD34+ hematopoietic progenitor cell differentiation.
Enabled local analysis of cytokine effects on hematopoietic stem cell recruitment, engraftment, and differentiation in four distinct tissue microenvironments.

Conclusions:

The developed model system provides a new platform for in vivo validation studies.
Facilitates the study of multiple human proteins and their local effects on hematopoietic cell biology.
Offers a powerful tool for advancing human stem cell research.