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MicroRNA‑335 inhibits bladder cancer cell growth and migration by targeting mitogen‑activated protein kinase 1

Xiaolin Wang1, Guang Wu2, Guangxin Cao1

  • 1Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.

Molecular Medicine Reports
|July 1, 2016
PubMed

Insights

MicroRNA-335 (miR-335) is downregulated in bladder cancer, acting as a tumor suppressor. Its reduced expression correlates with aggressive cancer, while restoring miR-335 inhibits proliferation and metastasis by targeting MAPK1.

Area of Science:

  • Molecular Oncology
  • Cancer Biology

Background:

  • MicroRNAs (miRs) play critical roles as oncogenes or tumor suppressors in various cancers.
  • The specific function of miR-335 in bladder cancer remains largely uncharacterized.

Purpose of the Study:

  • To investigate the expression levels of miR-335 in bladder cancer tissues.
  • To determine the impact of miR-335 on bladder cancer cell proliferation, apoptosis, and metastasis.
  • To identify potential molecular targets of miR-335 in bladder cancer.

Main Methods:

  • Quantitative PCR and Western blot analysis to assess miR-335 and MAPK1 expression.
  • MTT assay for cell proliferation assessment.
  • Flow cytometry for apoptosis analysis.
  • Transwell assay for cell migration evaluation.
  • Gene silencing techniques to validate target interactions.

Main Results:

  • miR-335 was significantly downregulated in bladder cancer tissues, with lower levels associated with aggressive disease phenotypes.
  • Overexpression of miR-335 inhibited T24 bladder cancer cell proliferation and induced apoptosis.
  • Restoring miR-335 levels suppressed cell migration and invasion.
  • Mitogen-activated protein kinase (MAPK)1 expression was reduced upon miR-335 overexpression, suggesting MAPK1 as a direct target.
  • Silencing MAPK1 mimicked the inhibitory effects of miR-335 on proliferation and migration.

Conclusions:

  • miR-335 functions as a tumor suppressor in bladder cancer.
  • The tumor-suppressive role of miR-335 is mediated, at least in part, through the repression of MAPK1.
  • miR-335 represents a potential therapeutic target for bladder cancer treatment.

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