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Compact Quantum Dots for Single-molecule Imaging
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Organic Dots Based on AIEgens for Two-Photon Fluorescence Bioimaging.

Xiaoding Lou1, Zujin Zhao2, Ben Zhong Tang2,3,4

  • 1School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

Small (Weinheim an Der Bergstrasse, Germany)
|July 1, 2016
PubMed
Summary

Aggregation-induced emission (AIE) fluorogens are used to create novel fluorescent organic dots for advanced two-photon imaging. These AIE-based dots offer superior brightness and stability for in vitro and in vivo bioimaging applications.

Keywords:
aggregation-induced emissionsbioimagingnanoparticlesorganic dotstwo-photon fluorescence

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Area of Science:

  • Biomedical Imaging
  • Materials Science
  • Nanotechnology

Background:

  • Two-photon fluorescence imaging offers high resolution and minimal photodamage.
  • Fluorescent organic dots are promising contrast agents for this technique.
  • Aggregation-caused quenching is a major limitation for conventional fluorophores in organic dots.

Purpose of the Study:

  • To highlight fluorescent organic dots utilizing aggregation-induced emission (AIE) fluorogens.
  • To discuss the potential of these AIE-based dots in bioimaging.
  • To address the challenge of fluorescence quenching in organic dot development.

Main Methods:

  • Synthesis of fluorescent organic dots with AIE fluorogens as emissive cores.
  • Characterization of the photophysical properties of the AIE-based dots.
  • Evaluation of the dots' performance in two-photon fluorescence imaging.

Main Results:

  • AIE fluorogens overcome aggregation-caused quenching, enabling strong fluorescence in aggregates.
  • Loading content of AIE fluorogen directly enhances two-photon absorption.
  • Demonstrated excellent performance in in vitro cellular imaging and in vivo vascular imaging of various tissues.

Conclusions:

  • Fluorescent organic dots based on AIEgens are highly effective for two-photon bioimaging.
  • These AIE-based dots exhibit significant potential for diverse bioimaging applications.
  • The use of AIEgens represents a significant advancement in developing bright and photostable organic dots.