Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Comprehensive Analysis of the Agreement and Performance of Variant Annotation Programs in Equine Genomes.

Genes·2026
Same author

Phloiokeratosis is a new ichthyosiform hyperkeratotic cornification disorder in dogs with SUV39H1 variants.

Scientific reports·2026
Same author

Reproducibility of the Evaluation of Genetic Variant Pathogenicity Based on the Animal Variant Classification Guidelines.

Animal genetics·2026
Same author

TG Nonsense Variant in Dwarf Rottweiler Dogs.

Animal genetics·2026
Same author

A canine <i>PLP1</i> missense variant differentiates oligodendrocyte maturation in connatal and classical Pelizaeus-Merzbacher disease.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

GJB6 missense variant in a Labrador Retriever with paw pad hyperkeratosis.

Animal genetics·2026

Related Experiment Video

Updated: Mar 18, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

7.9K

Optimized methods for extracting circulating small RNAs from long-term stored equine samples.

Lucia Unger1, Nathalie Fouché1, Tosso Leeb2

  • 1Department of Clinical Veterinary Medicine, Swiss Institute of Equine Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Länggassstrasse 124, 3012, Bern, Switzerland.

Acta Veterinaria Scandinavica
|July 1, 2016
PubMed
Summary
This summary is machine-generated.

Standardized methods for extracting circulating microRNAs (miRNAs) from equine samples are needed. This study demonstrates that high-quality miRNAs can be reliably recovered from equine serum and blood stored for over 10 years, enabling retrospective biomarker analysis.

Keywords:
EDTAHorseRNA extractionSerumSmall RNAmiRNAmicroRNA

More Related Videos

Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.2K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

29.0K

Related Experiment Videos

Last Updated: Mar 18, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

7.9K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.2K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

29.0K

Area of Science:

  • Veterinary Medicine
  • Biomarker Discovery
  • Molecular Diagnostics

Background:

  • Circulating microRNAs (miRNAs) in serum show potential as biomarkers for various diseases in both human and veterinary medicine.
  • Standardized methods for extracting miRNAs from equine serum and blood, especially from long-term archived samples, are currently lacking.
  • Reliable miRNA profiling requires robust and reproducible extraction techniques.

Purpose of the Study:

  • To systematically compare different miRNA extraction methods from equine serum and whole blood.
  • To assess the impact of storage duration and conditions on miRNA quality and integrity.
  • To establish a reliable method for recovering high-quality miRNAs from archived equine blood samples for Next-Generation Sequencing (NGS).

Main Methods:

  • Comparison of various miRNA extraction kits and protocols.
  • Analysis of miRNA yield and quality from equine serum and whole blood samples.
  • Evaluation of samples stored at room temperature for short periods before freezing.
  • Assessment of miRNA recovery from whole blood samples stored at -80°C for over 10 years.

Main Results:

  • Storage time at room temperature before freezing did not significantly impact miRNA quality in serum.
  • High-quality miRNAs suitable for NGS were successfully recovered from equine blood samples stored for more than 10 years at -80°C.
  • The study identified effective methods for miRNA extraction from challenging sample types.

Conclusions:

  • Standardized miRNA extraction protocols are crucial for reliable biomarker profiling in equine medicine.
  • Archived equine blood samples, even after prolonged storage, are a valuable resource for retrospective miRNA biomarker studies.
  • This research provides a foundation for developing robust methods for equine circulating miRNA analysis.