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Published on: October 15, 2014
Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
Timothy S Uphoff1, Blake W Buchan2, Nathan A Ledeboer2
1Molecular Pathology, Marshfield Labs, Marshfield, Wisconsin, USA uphoff.timothy@marshfieldclinic.org.
Abstract:
We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.
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