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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Profiling genome-wide DNA methylation.

Wai-Shin Yong1, Fei-Man Hsu2, Pao-Yang Chen1

  • 1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529 Taiwan, ROC.

Epigenetics & Chromatin
|July 1, 2016
PubMed
Summary
This summary is machine-generated.

This review overviews DNA methylation profiling techniques, essential for understanding gene regulation and diseases. It compares various assays, aiding researchers in selecting appropriate methods for epigenomic studies.

Keywords:
Bisulfite sequencingDNA methylationHydroxymethylationMethylomeRRBSSingle-cellWGBS

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Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • DNA methylation is a crucial epigenetic modification regulating gene expression, impacting biological processes and diseases.
  • Methylation patterns are tissue-specific, dynamic, and heritable, necessitating advanced profiling techniques.
  • Understanding DNA methylation is vital for biological research and disease mechanism elucidation.

Purpose of the Study:

  • To provide a comprehensive overview of major DNA methylation profiling techniques.
  • To compare different assays based on their strengths, weaknesses, and applications.
  • To guide researchers in selecting suitable methods for their specific epigenomic research questions.

Main Methods:

  • Survey of major experimental assays for DNA methylation and hydroxymethylation profiling.
  • Inclusion of genome-wide and single-cell resolution techniques.
  • Discussion of computational pipelines for bisulfite sequencing data analysis and differentially methylated region identification.

Main Results:

  • Advances in microarray and sequencing technologies enable genome-wide profiling at single-nucleotide or single-cell resolution.
  • Profiling approaches differ in DNA input, resolution, genomic coverage, and bioinformatics requirements.
  • Comparative analysis of platforms highlights their respective advantages and limitations.

Conclusions:

  • Selecting an appropriate DNA methylation profiling method requires understanding assay variations and research needs.
  • This review offers guidance on experimental and computational strategies for epigenomic profiling.
  • Effective profiling aids in answering complex biological questions related to gene regulation and disease.