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Tathagata Mondal¹, Jayita Sarkar¹, Suhrit Ghosh²

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Chemistry (Weinheim an Der Bergstrasse, Germany)

|July 1, 2016

Summary

We developed new amphiphilic block copolymers that self-assemble into robust nanoparticles. These nanoparticles exhibit aggregation-induced emission (AIE) and are suitable for long-term cancer cell tracking.

Keywords:

biosensors block copolymers fluorescence nanoparticles self-assembly

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Area of Science:

Polymer Chemistry
Materials Science
Nanotechnology

Background:

Amphiphilic block copolymers are crucial for self-assembly into nanostructures.
Aggregation-induced emission (AIE) is a photophysical phenomenon with potential applications in imaging and sensing.
Developing biocompatible nanomaterials for biological applications remains a key challenge.

Purpose of the Study:

To synthesize and characterize a novel ABA-type amphiphilic block copolymer.
To investigate the self-assembly behavior and nanoparticle formation of the synthesized polymer.
To evaluate the potential of these nanoparticles for biological imaging, specifically for long-term cancer cell tracking.

Main Methods:

Step-growth polymerization of tetraphenylethylene (TPE)-diol and hexamethylene di-isocyanate with polyethylene glycol (PEG).
Characterization of polymer self-assembly in water, including critical aggregation concentration (CAC).
Assessment of nanoparticle properties such as stability, biocompatibility (MTT assay), and aggregation-induced emission (AIE).
In vitro cellular imaging studies using cancer cells to evaluate cellular uptake and long-term tracking capabilities.

Main Results:

A novel ABA-type amphiphilic block copolymer was successfully synthesized in a single step.
The polymer self-assembles in water into robust nanoparticles driven by H-bonding and π-π interactions.
The resulting nanoparticles exhibit strong aggregation-induced emission (AIE), low CAC, good pH stability, and excellent biocompatibility.
Facile cellular uptake and long-term retention of AIE signals were observed in cancer cells, enabling effective long-term cell tracking.

Conclusions:

The developed amphiphilic block copolymer is a promising material for forming stable, AIE-active nanoparticles.
These nanoparticles demonstrate significant potential for advanced biological imaging and long-term tracking of cancer cells due to their biocompatibility and unique photophysical properties.