Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sinonasal microbiome remains stable during dupilumab therapy in chronic rhinosinusitis: a pilot study.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2026
Same author

Venous-trapezium coupler anastomosis in a partially congested combined ALT/TFL-flap: A case report.

JPRAS open·2026
Same author

Assessing coverage of the monitoring framework of the Kunming-Montreal Global Biodiversity Framework and opportunities to fill gaps.

Nature ecology & evolution·2025
Same author

[Registry Research Funding of the German Society of Plastic, Reconstructive and Aesthetic Surgery (DGPRÄC) and Research Funding Report 2021/2022].

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2023
Same author

[Accident-related and workplace-related vascular disorders of the hand].

Der Unfallchirurg·2022
Same author

[Registry Research Funding of the German Society of Plastic, Reconstructive and Aesthetic Surgeons (DGPRÄC) and Research Funding Report 2019/2020].

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2020
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

23.7K

Investigating non-specific binding to chemically engineered sensor surfaces using liposomes as models.

C Fenzl1, C Genslein, C Domonkos

  • 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstr. 31, 93053 Regensburg, Germany. antje.baeumner@ur.de.

The Analyst
|July 1, 2016
PubMed
Summary
This summary is machine-generated.

Non-specific binding in biosensors can be minimized by engineering nanoparticle and sensor surfaces. Tailoring surfaces with carboxyl (-COOH) or methyl (-CH3) groups significantly reduces unwanted adsorption, improving sensor performance.

More Related Videos

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

15.0K
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.3K

Related Experiment Videos

Last Updated: Mar 18, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

23.7K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

15.0K
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.3K

Area of Science:

  • Surface chemistry
  • Nanotechnology
  • Biosensor development

Background:

  • Non-specific binding (NSB) of nanoparticles hinders signal enhancement in biosensors.
  • Surface engineering of nanoparticles and sensor surfaces is crucial for minimizing NSB.
  • Understanding NSB mechanisms is key to designing effective biosensing systems.

Purpose of the Study:

  • To mechanistically characterize the non-specific binding of engineered liposomes on various sensor surfaces.
  • To investigate the impact of surface chemistry on liposome adsorption.
  • To identify optimal surface modifications for minimizing non-specific binding in biosensor applications.

Main Methods:

  • Utilized liposomes as model signal-enhancing nanoparticles with engineered surface charges.
  • Synthesized liposomes with varying carboxyl (-COOH) group densities.
  • Employed surface plasmon resonance (SPR) spectroscopy to quantify liposome-surface interactions.
  • Studied interactions with four chemically modified sensor surfaces, including methyl-, carboxyl-, and hydroxyl-terminated surfaces.

Main Results:

  • Non-specific binding followed Langmuir adsorption isotherms, providing quantitative binding data.
  • Hydrophilic alkane thiol-modified surfaces showed high correlation coefficients for NSB.
  • Carboxyl- (-COOH) and methyl- (-CH3) terminated sensor surfaces significantly minimized non-specific binding.
  • Pairing -COOH on liposomes with -COOH on sensor surfaces nearly eliminated NSB (1 mRIU at 100 μM).
  • Hydroxyl (-OH) terminated surfaces unexpectedly showed significant NSB (4 mRIU at 100 μM).

Conclusions:

  • Surface engineering of nanoparticles and sensor surfaces is critical for reducing non-specific binding.
  • Sensor surfaces terminated with -CH3 or -COOH groups are highly effective in minimizing liposome adsorption.
  • Strategic surface chemistry design can lead to improved biosensing systems with minimal non-specific adsorption.
  • This study provides a mechanistic understanding for optimizing nanoparticle-based biosensor design.