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A novel metabolite in the microbial degradation of 2-chlorobenzoate
S Fetzner1, R Müller, F Lingens
1Institut für Mikrobiologie der Universität Hohenheim, Stuttgart, FRG.
Biochemical and Biophysical Research Communications
|June 15, 1989
Abstract:
A bacterium which utilizes 2-chlorobenzoate as sole source of carbon and energy was isolated from water and tentatively identified as a Pseudomonas sp. During growth on 2-chlorobenzoate, the chlorine substituent was released quantitatively and a metabolite accumulated in the culture medium. Based on thin-layer chromatography, high-pressure liquid chromatography and gas chromatography linked to mass spectrometry this compound was unequivocally identified as 2,3-dihydroxybenzoate, a compound not described before in the degradation of 2-chlorobenzoate.