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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Advancements in Next-Generation Sequencing.

Shawn E Levy1, Richard M Myers1

  • 1HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806; email: slevy@hudsonalpha.org , rmyers@hudsonalpha.org.

Annual Review of Genomics and Human Genetics
|July 1, 2016
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) technologies provide high-output, genome-scale data. This review explores recent NGS advancements and their expanding applications driving genomic research progress.

Keywords:
exomesequencingwhole genome

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) has revolutionized biological research over the past decade.
  • NGS technologies enable high-throughput, genome-scale data production.
  • The applications and methodologies leveraging NGS have grown exponentially.

Purpose of the Study:

  • To review recent concepts and technologies in next-generation sequencing.
  • To illustrate the diverse applications and research areas advanced by NGS.
  • To highlight the progress driven by genome-scale sequencing.

Main Methods:

  • Literature review of recent advancements in next-generation sequencing.
  • Analysis of emerging technologies and methodologies.
  • Synthesis of application-driven progress in genomics.

Main Results:

  • Significant expansion in the scope and scale of genomic data generation.
  • Diverse applications across various biological research domains.
  • Identification of key trends and innovations in NGS.

Conclusions:

  • Next-generation sequencing continues to be a pivotal technology in modern genomics.
  • Ongoing advancements in NGS are accelerating discoveries across numerous research fields.
  • The future of genomics is intrinsically linked to the evolution of high-throughput sequencing methods.