Related Experiment Video
Updated: Mar 18, 2026
09:38
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method
Published on: December 1, 2015
15.7K
Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal
Atsushi Shoji1, Kana Ikeya1, Miki Aoyagi1
1Tokyo University Pharmacy and Life Science, 1432-1 Hachioji, Horinouchi, Tokyo 192-0392, Japan.
Journal of Pharmaceutical and Biomedical Analysis
|July 1, 2016
Abstract:
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a free 3-OH group in the β-configuration of sterols is required for recognition by SLO in a lipid bilayer. The extent of nanopore formation by SLO in lipid bilayers increased in the order of cholestanol
Keywords:
CholesterolCholesterol oxidaseFluorometric permeability assayImmobilized liposome membraneStreptolysin O
