Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-bridge Cycle01:26

Cross-bridge Cycle

124.4K
As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.
124.4K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

15.5K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety of Rapid Local Ischemic Postconditioning After Thrombectomy in Acute Stroke: A Dose-Finding Trial (RAPID SAVE).

Stroke·2026
Same author

Optimal scheduling study of microgrids based on multistrategy improved sardine algorithm.

Scientific reports·2026
Same author

Quadriceps mitochondrial DNA quantity, quality, and gene expression after 2 years of calorie restriction: exploratory results from the CALERIE trial.

GeroScience·2026
Same author

Brain frailty on penumbral salvage following successful reperfusion in MeVO stroke via collateral impairment.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026
Same author

Top-Down Proteomics of Skinned Human Muscle Fibers Reveals Proteoform-Resolved Fiber-to-Fiber Variability.

Journal of mass spectrometry : JMS·2026
Same author

Avidity-optimized TCR-T cells target KRAS neoantigens for potent cancer clearance and tumor microenvironment remodeling.

Frontiers in immunology·2026
Same journal

Proteomic Profiling of Endothelial Cells Under Laminar Shear Stress Confirms the Importance of KLF4 in the Regulation of Membrane Protein Expression Compared to Oscillatory Flow.

Journal of proteome research·2026
Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.8K

Top-Down Targeted Proteomics Reveals Decrease in Myosin Regulatory Light-Chain Phosphorylation That Contributes to

Zachery R Gregorich1,2, Ying Peng1, Wenxuan Cai1,2

  • 1Department of Cell and Regenerative Biology, University of Wisconsin-Madison , 1111 Highland Avenue, Madison, Wisconsin 53705, United States.

Journal of Proteome Research
|July 1, 2016
PubMed
Summary
This summary is machine-generated.

Aging causes sarcopenia, a loss of muscle function. This study found reduced myosin regulatory light chain (RLC) phosphorylation in aging muscles, explaining decreased muscle contractility and suggesting RLC phosphorylation as a therapeutic target.

Keywords:
agingmyofilamentsarcopeniatargeted proteomicstop-down mass spectrometry

More Related Videos

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
09:40

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Published on: January 19, 2017

12.4K
Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

13.0K

Related Experiment Videos

Last Updated: Mar 18, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.8K
Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
09:40

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Published on: January 19, 2017

12.4K
Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

13.0K

Area of Science:

  • Gerontology
  • Molecular Biology
  • Muscle Physiology

Background:

  • Sarcopenia, age-related muscle mass and function loss, is a major cause of disability in the elderly.
  • The molecular basis of age-related muscle dysfunction in sarcopenia is not fully understood.
  • Myosin regulatory light chain (RLC) phosphorylation is crucial for muscle contractility.

Purpose of the Study:

  • To investigate the molecular mechanisms of age-related muscle dysfunction in sarcopenia.
  • To identify changes in protein phosphorylation associated with aging muscle.
  • To correlate RLC phosphorylation levels with muscle contractile properties.

Main Methods:

  • Utilized top-down targeted proteomics and mechanical measurements in aging rat skeletal muscle.
  • Performed top-down tandem mass spectrometry to analyze RLC proteoforms and phosphorylation sites.
  • Conducted mechanical analysis on single fast-twitch muscle fibers from rats of varying ages.

Main Results:

  • Identified a progressive age-related decline in RLC phosphorylation in skeletal muscle.
  • Discovered a novel bis-phosphorylated RLC proteoform and pinpointed decreasing phosphorylation at Ser14/15.
  • Demonstrated that reduced RLC phosphorylation correlates with diminished contractile function in sarcopenic fast-twitch muscles.

Conclusions:

  • Decreased RLC phosphorylation is a key factor in age-related sarcopenia and muscle dysfunction.
  • Ser14 phosphorylation on RLC is a previously unrecognized site in fast-twitch skeletal muscle.
  • Modulating RLC phosphorylation presents a potential therapeutic strategy for treating sarcopenia.