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Interactions between native oat phytochrome and tetrapyrroles.

B R Singh1, P S Song

  • 1Department of Chemistry, School of Biological Sciences, University of Nebraska, Lincoln 68588.

Biochimica Et Biophysica Acta
|June 13, 1989
PubMed
Summary
This summary is machine-generated.

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The study investigated how tetrapyrroles affect phytochrome

Area of Science:

  • Biochemistry
  • Plant Photobiology
  • Spectroscopy

Background:

  • Phytochrome's Pr to Pfr phototransformation involves a change in its far-UV CD signal.
  • This change was hypothesized to result from chromophore interaction with the N-terminus.

Purpose of the Study:

  • To investigate the role of exogenous tetrapyrroles in phytochrome's phototransformation.
  • To examine chromophore-protein interactions during Pr to Pfr conversion.

Main Methods:

  • Circular dichroism (CD) spectroscopy was used to measure changes in phytochrome.
  • Exogenous tetrapyrroles (bilirubin, biliverdin, chlorophyllin, hemin) were added to oat phytochrome.
  • Secondary structure analysis was performed.

Main Results:

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  • Open tetrapyrroles had no significant effect on phototransformability.
  • Closed tetrapyrroles blocked the increase in the far-UV CD signal during Pr to Pfr transformation.
  • Chlorophyllin increased alpha-helix content in both Pr and Pfr forms.
  • Hemin caused bleaching of the Pfr form.

Conclusions:

  • Tetrapyrrole type influences chromophore-protein interactions in phytochrome.
  • Closed tetrapyrroles interfere with the conformational changes associated with Pr to Pfr phototransformation.
  • The study provides insights into the altered chromophore-protein interactions during phytochrome signaling.