Heat shock stress is deleterious to CNS cultured neurons microinjected with anti-HSP70 antibodies

CNS cultured neurons microinjected with anti-mouse immunoglobulin or anti-hsp70 antibodies and cultured at 37 degrees C remained healthy while those injected with anti-hsp70 antibodies, exposed to 50 degrees C for 15 min and returned to 37 degrees C exhibited deleterious effects i.e. lesser interconnecting neurites and poor neuronal population. This shows that heat shock stress affects the survival of neurons in vitro.