Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.5K
CRISPR01:59

CRISPR

58.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.8K
CRISPR01:59

CRISPR

18.8K
18.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.5K
Homologous Recombination02:31

Homologous Recombination

65.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic imaging of <i>Fragilariopsis cylindrus</i> in polar night conditions using full-field optical transmission tomography (FFOTT).

Biomedical optics express·2026
Same author

Tryptophanol enhances nitrogen assimilation in marine diatoms.

Nature communications·2026
Same author

[Resolving Genomic Mysteries with Long-read Sequencing].

Harefuah·2026
Same author

Coral microbiomes as reservoirs of unknown genomic and biosynthetic diversity.

Nature·2026
Same author

Markedly Low Prevalence of Fatty Liver Despite Obesity in Prader-Willi Syndrome: A Search for Protective Genetic Markers.

Journal of clinical and experimental hepatology·2026
Same author

Genomic features and alkane-metabolism profiles of three new Alcanivoracaceae bacteria isolates from the Eastern Mediterranean Sea.

Marine pollution bulletin·2025

Related Experiment Video

Updated: Mar 18, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.4K

PhytoCRISP-Ex: a web-based and stand-alone application to find specific target sequences for CRISPR/CAS editing.

Achal Rastogi1, Omer Murik1, Chris Bowler1

  • 1Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, PSL Research University, CNRS UMR 8197, INSERM U1024, 46 rue d'Ulm, F-75005, Paris, France.

BMC Bioinformatics
|July 2, 2016
PubMed
Summary

PhytoCRISP-Ex is a new tool for CRISPR target prediction in phytoplankton genomes. It offers high sensitivity and a user-friendly interface for efficient genome editing research.

Keywords:
CRISPRCas9EukaryotesGenome editingProtists

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.2K

Related Experiment Videos

Last Updated: Mar 18, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.4K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.2K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Growing interest in phytoplankton research necessitates advanced genetic tools for gene functional characterization.
  • CRISPR/Cas9 is a powerful genome editing system, but existing tools inadequately support non-model organisms like protists.
  • Current computational tools for CRISPR target prediction lack comprehensive databases for phytoplankton genomes.

Purpose of the Study:

  • To develop PhytoCRISP-Ex, a novel computational tool for CRISPR target prediction.
  • To provide a user-friendly platform for genome editing in phytoplankton and other non-model organisms.
  • To enhance the efficiency and accuracy of CRISPR-based functional genomics in diverse species.

Main Methods:

  • Development of a web-based platform integrating extensive phytoplankton genome data.
  • Creation of a standalone application for universal genome compatibility.
  • Implementation of sensitive Cas9 target prediction algorithms.
  • Integration of restriction site analysis for efficient mutant screening.

Main Results:

  • PhytoCRISP-Ex provides high-sensitivity Cas9 target predictions across numerous phytoplankton genomes.
  • The software offers a user-friendly web interface and a versatile standalone application.
  • PhytoCRISP-Ex outperforms existing tools by incorporating restriction site availability at cleavage sites.

Conclusions:

  • PhytoCRISP-Ex is a fast, accurate, and user-friendly tool for CRISPR target identification.
  • The tool supports 13 pre-indexed phytoplankton genomes and is extensible to other species.
  • PhytoCRISP-Ex facilitates efficient genome editing and mutant screening in diverse research pipelines.