Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

1.3K
Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
1.3K
Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

4.4K
Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
Same author

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same author

Reviving formalin-fixed, paraffin embedded (FFPE) tissues for on-slide and multiscale correlative microscopy.

Scientific reports·2026
Same author

Non-invasive electrical stimulation restores corneal nerve density and sensory function in diabetic neuropathy via KCNN-dependent mechanism.

Communications biology·2026
Same author

Best Practice Imaging in the Initial Investigation of Paediatric Blunt Abdominal Trauma: A Narrative Review.

Journal of medical radiation sciences·2026
Same author

Mixed gangliocytoma-pituitary neuroendocrine tumour: clinical, immunohistochemical, and molecular genetic profiles in a series of four patients.

Acta neuropathologica communications·2026
Same journal

Light-Induced Proteomic Changes in Pseudomonas aeruginosa Biofilms.

Proteomics·2026
Same journal

Decade-Resolved Proteomic Profiling of Gastric Cancer FFPE Archives: Evaluating Storage-Associated Shifts and Signal Stability Over 50 Years.

Proteomics·2026
Same journal

Proteome-Scale Mining of Metal-Associated Proteins of Monkeypox Virus.

Proteomics·2026
Same journal

Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.

Proteomics·2026
Same journal

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus-Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System.

Proteomics·2026
Same journal

Functional Divergence of Mucus in Pacific Oyster (Crassostrea gigas): Insights From Integrated Proteomic and Rheological Study.

Proteomics·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.6K

Tissue microarray profiling in human heart failure.

Sean Lal1, Lisa Nguyen2, Rhenan Tezone2

  • 1Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, The University of Sydney, Sydney, Australia. sean@anatomy.usyd.edu.au.

Proteomics
|July 2, 2016
PubMed
Summary
This summary is machine-generated.

This study developed a novel Tissue MicroArray (TMA) system using human heart tissues to analyze protein expression in heart failure. Researchers found significantly reduced FHL2 protein levels in human heart failure samples.

Keywords:
CardiomyopathyHuman heart failureLim proteinsProtein arraysProteomic profilingTissue microarray

More Related Videos

3D Whole-heart Myocardial Tissue Analysis
06:53

3D Whole-heart Myocardial Tissue Analysis

Published on: April 12, 2017

9.4K
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

18.2K

Related Experiment Videos

Last Updated: Mar 18, 2026

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.6K
3D Whole-heart Myocardial Tissue Analysis
06:53

3D Whole-heart Myocardial Tissue Analysis

Published on: April 12, 2017

9.4K
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

18.2K

Area of Science:

  • Cardiovascular Biology
  • Biotechnology
  • Molecular Pathology

Background:

  • Tissue MicroArrays (TMAs) enable high-throughput protein screening on single slides.
  • Cryopreserved human cardiac samples from heart failure and donor patients are valuable for molecular studies.
  • The role of four-and-a-half LIM-domain 2 (FHL2) in human heart failure pathogenesis is not well understood.

Purpose of the Study:

  • To develop a customizable TMA system using cryopreserved human cardiac tissues.
  • To investigate the expression of FHL2 protein in human heart failure.
  • To establish a method for high-throughput protein profiling in cardiac diseases.

Main Methods:

  • Development of a novel TMA system utilizing cryopreserved human cardiac samples.
  • Generation of an affinity-purified rabbit polyclonal anti-human FHL2 antibody.
  • Application of TMAs for qualitative and semiquantitative immunohistochemistry of FHL2.
  • Complementary analysis using Western blot.

Main Results:

  • The TMA system successfully produced formalin-fixed paraffin-embedded sections from cryopreserved cardiac tissues.
  • High-throughput profiling of FHL2 protein expression was achieved using immunohistochemistry on TMAs.
  • A significant relative reduction in FHL2 protein expression was observed in human heart failure samples compared to controls.

Conclusions:

  • The developed TMA system is a versatile tool for high-throughput protein analysis in cardiac research.
  • FHL2 protein expression is significantly reduced in human heart failure.
  • This study provides insights into the molecular mechanisms of human heart failure.