Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.1K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.8K
3.8K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.8K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.8K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.8K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.8K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.3K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.8K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell Therapy From Bench to Bedside Translation in CNS Neurorestoratology Era.

Cell medicine·2011
Same author

Enhancing the Curie temperature of ferromagnetic semiconductor (Ga,Mn)As to 200 K via nanostructure engineering.

Nano letters·2011
Same author

Anticancer activity of 2α, 3α, 19β, 23β-Tetrahydroxyurs-12-en-28-oic acid (THA), a novel triterpenoid isolated from Sinojackia sarcocarpa.

PloS one·2011
Same author

Gorilla genome structural variation reveals evolutionary parallelisms with chimpanzee.

Genome research·2011
Same author

Activation of presynaptic GABAB receptors modulates GABAergic and glutamatergic inputs to the medial geniculate body.

Hearing research·2011
Same author

Effect of transplantation with autologous bone marrow stem cells on acute myocardial infarction.

International journal of cardiology·2011
Same journal

Correction to: Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Deletion of CEACAM1 does not affect retinal and choroidal morphology or transcriptome.

Cell and tissue research·2026
Same journal

Cardiac α2δ1 C-terminal contributes to left atrial hypertrophy in chronic ischemic heart failure, in association with changes in membrane GluN1 and p-CAMKII/p-HDAC4 signaling.

Cell and tissue research·2026
Same journal

Gill ionocytes of the Lake Magadi tilapia (Oreochromis Alcolapia grahami), an extremophilic teleost native to a highly alkaline environment.

Cell and tissue research·2026
Same journal

Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Effects of gestational protein restriction on autophagy dynamics during odontogenesis.

Cell and tissue research·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K

Long noncoding RNAs in cell differentiation and pluripotency.

Lin Chen1, Sheng Zhang2

  • 1Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, People's Republic of China. drlc2011@163.com.

Cell and Tissue Research
|July 2, 2016
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) are key regulators of gene expression, impacting cell differentiation and pluripotency. This review details their mechanisms and roles in these vital biological processes.

Keywords:
Cell differentiationGene expressionLong noncoding RNAsPluripotencyRegulation

More Related Videos

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.0K
Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

458

Related Experiment Videos

Last Updated: Mar 18, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.0K
Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

458

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Long noncoding RNAs (lncRNAs) were initially dismissed as transcriptional noise.
  • Emerging evidence highlights their critical roles in gene regulation and cellular functions.
  • Understanding lncRNA complexity is essential for deciphering their biological significance.

Purpose of the Study:

  • To review the biological characteristics and regulatory mechanisms of lncRNAs.
  • To discuss the role of lncRNAs in cell differentiation.
  • To explore the function of lncRNAs in maintaining pluripotency.

Main Methods:

  • Literature review of recent studies on lncRNA functions.
  • Analysis of lncRNA regulatory mechanisms at multiple levels.
  • Synthesis of current knowledge on lncRNAs in cell differentiation and pluripotency.

Main Results:

  • lncRNAs regulate gene expression through chromatin remodeling, transcriptional, and post-transcriptional mechanisms.
  • Specific lncRNAs have been identified as crucial players in directing cell fate.
  • lncRNAs are integral to the maintenance of embryonic stem cell pluripotency.

Conclusions:

  • lncRNAs are fundamental regulators in cell biology, particularly in differentiation and pluripotency.
  • Further research into lncRNA mechanisms will deepen our understanding of gene regulation.
  • lncRNAs represent a significant area for future biological and medical research.