Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker

Area of Science:

• Phytochemistry
• Analytical Chemistry
• Pharmacognosy

Background:

• Traditional medicines require robust quality control methods.
• Linderae Reflexae Radix contains valuable bioactive compounds.
• Accurate quantification of multi-components is essential for herbal drug standardization.

Purpose of the Study:

• To establish and validate the quantitative analysis of multi-components by the single marker (QAMS) method.
• To assess the feasibility of QAMS for quality evaluation of Linderae Reflexae Radix.
• To simultaneously quantify four main components in Linderae Reflexae Radix.

Main Methods:

• Quantitative analysis of multi-components by the single marker (QAMS) method.
• Reverse-phase high-performance liquid chromatography (RP-HPLC) with UV detection.
• Comparison with the external standard method using different HPLC systems and columns.

Main Results:

• The QAMS method demonstrated high accuracy and reliability for quantifying four main components in Linderae Reflexae Radix.
• No significant differences (RSD <3%) were observed between QAMS and the external standard method.
• Pinosylvin was effectively used as a single marker for the simultaneous quantitative assay of pinostrobin, pinosylvin, pinocembrin, and Reflexanbene I.

Conclusions:

• The QAMS method is a feasible and reliable approach for the quality control of Linderae Reflexae Radix.
• This method simplifies multi-component analysis and reduces the need for multiple reference standards.
• QAMS offers a valuable tool for ensuring the consistency and efficacy of herbal medicines.