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Histopathological changes in retinas and F-ERG features of streptozotocin-induced diabetic rats treated with ozone
Ting-Yu Xie1, Qin Li1, Xue-Yi Chen1
1Department of Ophthalmology, the First Affiliated Hospital of Xinjiang Medical University, Urumchi 830011, Xinjiang Uygur Autonomous Region, China.
Aim:
To study the histopathological changes in the retina and flash electroretinogram (F-ERG) features of ozone-treated streptozotocin (STZ)-induced diabetic rats.
Methods:
Seventy male Sprague Dawley rats were grouped as follows: blank group (GB, n=10), model control group (GM, n=18), ozone group (GO3, n=19), and oxygen group (GO2, n=18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment.
Results:
Under dark adaption, as compared to GB, the other groups each had differential decreases in the a-wave amplitudes (P<0.05); the latencies were delayed in GM, GO2, and GO3 rats (P<0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes (F=0.28, P>0.05). There were significant differences in the apoptosis index among the groups (P<0.05). Under ozone treatment, apoptosis was decreased in GO3 as compared to GM and GO2.
Conclusion:
Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.
