Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implantation of a Continuous-Flow Left Ventricular Assist Device During Cardiopulmonary Bypass Is Associated with a Significant and Transient Acute Thromboinflammatory Response.

International journal of molecular sciences·2026
Same author

Native C3 is activated without proteolytic cleavage by transformation to C3(H<sub>2</sub>O) on phospholipid-scrambled cell membranes.

Frontiers in immunology·2026
Same author

Targeting the apical domain of the transferrin receptor: Development of a new protein scaffold for cellular delivery.

Protein science : a publication of the Protein Society·2025
Same author

Perceived stress levels among patients treated for neovascular age-related macular degeneration with anti-VEGF injections.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2025
Same author

Complement C3 inhibition reduces complement activation in clinical platelet concentrates but does not counteract platelet storage lesions.

Platelets·2025
Same author

Qualitative evaluations of reactive microglial heterogeneity in cultured porcine retina.

Histology and histopathology·2024

Related Experiment Video

Updated: Mar 18, 2026

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
09:46

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Published on: February 26, 2021

3.7K

Human neural progenitor cells decrease photoreceptor degeneration, normalize opsin distribution and support synapse

Tanzina Mollick1, Camilla Mohlin2, Kjell Johansson1

  • 1School of Health and Medicine, Örebro University, Sweden.

Brain Research
|July 3, 2016
PubMed
Summary
This summary is machine-generated.

Human neural progenitor cells (hNPCs) protected photoreceptors and supported retinal cell survival in explant models of neurodegenerative disorders. These findings suggest hNPC-derived factors may offer a novel therapeutic strategy for preserving vision.

Keywords:
GliosisNeuroprotectionOpsinPhotoreceptor degenerationSynapse

More Related Videos

Organotypic Culture of Full-thickness Adult Porcine Retina
08:17

Organotypic Culture of Full-thickness Adult Porcine Retina

Published on: March 20, 2011

15.5K
Primary Culture of Porcine Retinal Pigment Epithelial Cells
07:59

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Published on: September 23, 2022

3.0K

Related Experiment Videos

Last Updated: Mar 18, 2026

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
09:46

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Published on: February 26, 2021

3.7K
Organotypic Culture of Full-thickness Adult Porcine Retina
08:17

Organotypic Culture of Full-thickness Adult Porcine Retina

Published on: March 20, 2011

15.5K
Primary Culture of Porcine Retinal Pigment Epithelial Cells
07:59

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Published on: September 23, 2022

3.0K

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Regenerative Medicine

Background:

  • Retinal neurodegenerative diseases cause vision loss through photoreceptor and neuronal cell death.
  • Current treatments for these conditions are limited, highlighting the need for novel therapeutic approaches.

Purpose of the Study:

  • To investigate the potential of human neural progenitor cell (hNPC) derived factors to mitigate degenerative processes in porcine retinal explants.
  • To evaluate the protective effects of hNPCs on photoreceptors, synaptic integrity, and neuronal survival.

Main Methods:

  • Porcine retinal explants were cultured with or without hNPCs.
  • Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was used to assess cell death.
  • Immunohistochemistry, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to analyze cellular and molecular changes.

Main Results:

  • hNPCs significantly limited photoreceptor cell death and improved the maintenance of cone outer segments.
  • Synaptic structural integrity was preserved, and calbindin-positive horizontal cells showed enhanced survival.
  • Müller cell gliosis was reduced in terms of cell density, though not fully eliminated.

Conclusions:

  • hNPC-derived factors demonstrate a capacity to protect photoreceptors, maintain synaptic integrity, and support horizontal cell survival in retinal explants.
  • hNPC-based treatments may represent a promising strategy for preserving retinal function in neurodegenerative diseases.
  • Further research is needed to determine optimal hNPC concentrations or combinations for fully addressing Müller cell gliosis.