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Excimer fluorescence in lens proteins labelled with N-(1-pyrene)maleimide
1Russell Grimwade School of Biochemistry, Melbourne University, Victoria, Australia.
Current Eye Research
|May 1, 1989
Abstract:
Lens proteins labelled with the sulphydryl reagent, N-(1-pyrene)maleimide exhibit pyrene excimer fluorescence around 470 nm. This was found to be associated with both the beta- and gamma-crystallins but not the alpha-crystallins. Disappearance of the 470 nm peak on exposure to denaturants indicates it arose from an intramolecular excimer. This excimer fluorescence provides a new and sensitive tool for monitoring conformational changes in the beta- and gamma-crystallins.