Related Experiment Video
Updated: Mar 18, 2026
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
Comparison between Pentacam-HR and optical coherence tomographycentral corneal thickness measurements in healthy
Allison M Cleymaet1, Ann M Hess2, Kate S Freeman1
1Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, Veterinary Teaching Hospital, 300 West Drake Road, Fort Collins, CO, 80523, USA.
Objectives:
To measure the central corneal thickness (CCT) in healthy feline eyes with Scheimpflug imaging (Pentacam, Pentacam(®) -HR) and to compare these values with those obtained with spectral-domain optical coherence tomography (OCT, Optovue(®) iVue).
Animals Studied:
Thirty one sedated Domestic Short-haired cats.
Procedures:
Two repeated CCT measurements were obtained from both eyes using Pentacam measured at the pupil center and corneal apex (CCTpupil and CCTapex ) and using SD-OCT (CCTOCT ). Agreement between the imaging modalities for CCT and intradevice repeatability was evaluated with Bland-Altman analysis. Mixed modeling was used to test for a difference between methods.
Results:
The calculated mean ± SD CCT was CCTOCT = 584.93 ± 39.05 μm, CCTpupil = 608.25 ± 47.26 μm, and CCTapex = 606.41 ± 44.18 μm. There was a statistically significant difference between CCTOCT vs. CCTpupil (P < 0.0007) and CCTOCT vs. CCTapex (P < 0.0003) (n = 59 eyes). The 95% limits of agreement (LoA) for CCTOCT vs. CCTpupil was (-89.57 μm, 40.04 μm) and for CCTOCT vs. CCTapex was (-79.62 μm, 33.79 μm) (n = 59 eyes). 95% LoA between repeated CCT measurements by SD-OCT was (-10.23 μm, 9.32 μm) and by Pentacam was (-22.66 μm, 18.30 μm) at pupil and (-15.81 μm, 16.17 μm) at corneal apex (n = 11 eyes).
Conclusions:
SD-OCT and Pentacam provide excellent CCT measurement repeatability. Pentacamapex is our recommended clinical setting for use of the Pentacam. The level of agreement between SD-OCT and Pentacam for CCT is good.
