Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

3.3K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
3.3K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

6.7K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
6.7K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

5.8K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
5.8K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

2.8K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infrared Spectroscopic Signatures of the Fluorous Effect Arise from a Change of Conformational Dynamics.

Journal of the American Chemical Society·2025
Same author

Evaluating aliphatic CF, CF2, and CF3 groups as vibrational Stark effect reporters.

The Journal of chemical physics·2024
Same author

Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization.

Structural dynamics (Melville, N.Y.)·2016
Same author

High-Field High-Repetition-Rate Sources for the Coherent THz Control of Matter.

Scientific reports·2016
Same author

The use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment.

Letters in applied microbiology·2009
Same author

Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America·2008

Related Experiment Video

Updated: Mar 18, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

679

New ultrarapid-scanning interferometer for FT-IR spectroscopy with microsecond time-resolution.

B Süss1, F Ringleb2, J Heberle1

  • 1Department of Physics, Experimental Molecular Biophysics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.

The Review of Scientific Instruments
|July 3, 2016
PubMed
Summary

A new Fourier-transform infrared spectrometer offers 1000x faster scans using a novel sonotrode design. This breakthrough enables detailed study of rapid biological processes in solution, like protein photoreactions.

More Related Videos

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

34.0K
Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

6.7K

Related Experiment Videos

Last Updated: Mar 18, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

679
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

34.0K
Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

6.7K

Area of Science:

  • Spectroscopy
  • Biophysics
  • Analytical Chemistry

Background:

  • Conventional rapid-scanning spectrometers have limitations in time resolution.
  • Studying fast biological processes in solution requires advanced spectroscopic techniques.

Purpose of the Study:

  • To develop a novel Fourier-transform infrared (FT-IR) rapid-scan spectrometer with significantly enhanced time resolution.
  • To demonstrate the spectrometer's capability in capturing fast biological reactions in solution.

Main Methods:

  • Development of a novel FT-IR spectrometer utilizing a sonotrode as the movable interferometer mirror.
  • Achieved a time resolution of 13 μs with automated long-term measurements.
  • Recorded the photoreaction of bacteriorhodopsin using synchronized laser pulses and FT-IR data acquisition.

Main Results:

  • The novel spectrometer achieves 1000 times higher time resolution compared to conventional systems.
  • Successfully recorded fast photoreactions of bacteriorhodopsin in solution.
  • Demonstrated superior performance over step-scan spectroscopy for certain applications.

Conclusions:

  • The developed FT-IR spectrometer enables high-time-resolution studies of fast, non-repetitive processes in solution.
  • This technology is crucial for investigating dynamic events in biological systems, such as protein dynamics.
  • Opens new avenues for research in biophysics and molecular mechanisms.