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G-Protein Gated Ion Channels

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GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...

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Related Experiment Video

Updated: Mar 18, 2026

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

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Published on: September 14, 2016

Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents (Ih) in Neurons.

¹Department of Pharmacology, UCL School of Pharmacy, University College London, London, WC1N 1AX, United Kingdom.

Cold Spring Harbor Protocols

|July 3, 2016

Summary

This study details voltage-clamp methods for recording neuronal hyperpolarization-activated cyclic nucleotide-gated (HCN) channel currents (Ih). Understanding Ih properties is vital for neurological research and treating disorders like epilepsy and depression.

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Area of Science:

Neuroscience
Molecular Biology
Physiology

Background:

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are crucial for physiological processes like memory and navigation.
Dysfunction of HCN channels is linked to neurological disorders such as epilepsy and depression.
Neuronal HCN currents (Ih) exhibit diverse biophysical properties due to subunit heterogeneity, impacting neuronal activity.

Purpose of the Study:

To describe voltage-clamp methods for directly recording neuronal Ih.
To enable detailed analysis of Ih biophysical properties in individual neurons.
To facilitate understanding of Ih's role in neuronal function and disease.

Main Methods:

Whole-cell voltage-clamp recordings.
Cell-attached patch-clamp recordings.
Outside-out patch-clamp recordings.

Main Results:

Provides a detailed protocol for recording neuronal Ih.
Enables characterization of Ih biophysical properties.
Offers data applicable to computational modeling.

Conclusions:

Direct recording of Ih is essential for understanding its diverse roles.
This protocol supports research into HCN channel function and dysfunction.
Findings can advance therapeutic strategies for neurological disorders.