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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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Optimization of a single phase method for lipid extraction from milk.

Zhiqian Liu1, Simone Rochfort2, Benjamin G Cocks2

  • 1Biosciences Research, Department of Economic Development, Jobs, Transport and Resources, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia.

Journal of Chromatography. A
|July 4, 2016
PubMed
Summary

A new one-phase solvent system simplifies milk lipid extraction for liquid chromatography-mass spectrometry (LC-MS) lipidomics. This method offers comprehensive lipid recovery, improving high-throughput analysis of milk samples.

Keywords:
LipidLiquid chromatography–mass spectrometryMilkOne-phase extraction

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Food Science

Background:

  • Liquid chromatography-mass spectrometry (LC-MS) is crucial for milk lipidomic analysis.
  • Traditional Folch and Bligh and Dyer methods for milk lipid extraction are time-consuming due to their two-phase nature.

Purpose of the Study:

  • To develop and evaluate a novel one-phase solvent system for efficient milk lipid extraction.
  • To compare the performance of the new method against the standard Folch extraction technique.

Main Methods:

  • Comparison of three one-phase solvent systems with the standard Folch method for total lipid extraction from raw milk.
  • Evaluation of extraction efficiency for polar lipids and triglycerides using LC-MS.

Main Results:

  • Two tested butanol/methanol solvent systems showed incomplete triglyceride extraction.
  • A newly designed butanol/methanol/chloroform (3:5:4) solvent mix demonstrated comparable triglyceride extraction efficiency to the Folch method.
  • The new one-phase method yielded higher recovery for certain phospholipids compared to the Folch method.

Conclusions:

  • The developed one-phase extraction method is simple, comprehensive, and efficient for milk lipid analysis.
  • This method is suitable for high-throughput lipidomic studies of milk samples, overcoming limitations of traditional two-phase extraction techniques.