Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Duplication of Chromatin Structure

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and Validation of a Cross-Dimensional Screening Protocol for Remote Phenotyping Applied to Individuals With 3q29 Deletion Syndrome.

Journal of intellectual disability research : JIDR·2026

Same author

CFTR modulators-induced acne: Case series and analysis of pharmacovigilance databases.

Annales de dermatologie et de venereologie·2026

Same author

Lifestyle behaviours can mediate socio-economic and sex inequalities in children's oral health: findings from Children's Dental Health Survey 2013, England, Wales, and Northern Ireland.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2026

Same author

[Application and process optimization of automated magnetic bead sorting technology in T-SPOT.TB assay for tuberculosis infection].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025

Same author

Peroxisome Functional Inhibition Alleviates TMJOA Cartilage Degradation.

Journal of dental research·2025

Same author

Reproductive Journeys: A Comparative Analysis of Patient-Centered Care and Well-Being in 2S/LGBTQIA+ and Mixed-Sex/Gender Couples Undergoing Medically Assisted Reproduction.

Journal of homosexuality·2025

Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026

Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026

Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026

Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026

Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026

Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 18, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

In Vitro Chromatin Assembly: Strategies and Quality Control.

U Muthurajan¹, F Mattiroli¹, S Bergeron¹

¹University of Colorado at Boulder, Boulder, CO, United States.

Methods in Enzymology

|July 4, 2016

Summary

This summary is machine-generated.

High-quality chromatin reconstitution is crucial for understanding gene regulation. This chapter details methods for in vitro chromatin assembly and quality control, ensuring reliable experimental results.

Keywords:

Analytical ultracentrifugation Atomic force microscopy Fluorescence labeling Histone Micrococcal nuclease digestion Native polyacrylamide gel electrophoresis Nucleosomal array Nucleosome SEC-MALS Small-angle X-ray scattering

More Related Videos

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Published on: November 17, 2023

Related Experiment Videos

Last Updated: Mar 18, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Published on: November 17, 2023

Area of Science:

Molecular Biology
Epigenetics
Chromatin Biology

Background:

Chromatin structure dictates access to genetic and epigenetic information.
Regulatory proteins orchestrate chromatin structural transitions for nuclear processes.
In vitro chromatin reconstitution is vital for mechanistic studies.

Purpose of the Study:

To describe methods for in vitro chromatin reconstitution.
To outline quality control procedures for chromatin substrates.
To ensure reproducibility and reliability in chromatin-based experiments.

Main Methods:

Detailed protocols for in vitro chromatin assembly using defined components.
Quality control assays to assess chromatin homogeneity and integrity.
Comparative analysis of different reconstitution and quality control approaches.

Main Results:

Established robust methods for producing high-quality, quality-controlled chromatin substrates.
Highlighted critical quality control steps for reliable chromatin preparations.
Identified strengths and weaknesses of various reconstitution techniques.

Conclusions:

Standardized in vitro chromatin reconstitution and quality control are essential.
Reliable chromatin substrates enable accurate mechanistic investigations.
This work facilitates reproducible research in chromatin biology.