Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.1K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.8K
3.8K
Types of RNA01:20

Types of RNA

15.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
15.6K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
RNA-seq03:21

RNA-seq

12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circ_015350 Mediates Odonto/osteogenic Differentiation in Different Stem Cells.

Journal of dental research·2026
Same author

Influence of water temperature on feed intake, appetite control, and energy allocation in Atlantic salmon (<i>Salmo salar</i>) post-smolt.

Frontiers in physiology·2025
Same author

A novel missense variant of <i>FBN1</i> gene in a Sardinian family with Marfan syndrome: a case report.

Frontiers in pediatrics·2025
Same author

Demonstrating the reliability of in vivo metabolomics based chemical grouping: towards best practice.

Archives of toxicology·2024
Same author

Comparison of Pulmonary and Extrapulmonary Models of Sepsis-Associated Acute Lung Injury.

Physiological research·2024
Same author

Mode of action hypothesis testing in chemical safety assessments using metabolomics as supporting evidence: Phenobarbital and cyclobutrifluram metabolomics profile comparison.

Toxicology letters·2023
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.7K

Detection and Analysis of Long Noncoding RNAs.

F Lai1, E Blumenthal1, R Shiekhattar1

  • 1Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, United States.

Methods in Enzymology
|July 4, 2016
PubMed
Summary
This summary is machine-generated.

This review explores how long noncoding RNAs (lncRNAs) function in gene regulation by mapping them to genomic regulatory elements. It details high-throughput sequencing methods for analyzing these crucial RNA molecules.

Keywords:
Cap analysis of gene expressionGlobal run-on sequencingLong noncoding RNANext-generation sequencingPhotoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitationRNA immunoprecipitation and sequencing

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.4K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

24.5K

Related Experiment Videos

Last Updated: Mar 18, 2026

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.7K
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.4K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

24.5K

Area of Science:

  • Genomics
  • Molecular Biology
  • RNA Biology

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their regulatory roles in eukaryotic genomes.
  • Genome-wide studies show lncRNAs frequently localize to promoter and enhancer elements, suggesting involvement in gene regulation.
  • Understanding lncRNA function requires robust analytical methodologies.

Purpose of the Study:

  • To review current high-throughput sequencing methodologies for analyzing long noncoding RNA (lncRNA) function.
  • To provide an overview of techniques for investigating lncRNA interactions with regulatory genomic elements.
  • To highlight specific protocols for comprehensive lncRNA analysis.

Main Methods:

  • Direct RNA sequencing for precise lncRNA identification.
  • RNA sequencing coupled with transcription assays to capture dynamic lncRNA expression.
  • Isolation of lncRNA-associated protein complexes followed by high-throughput sequencing (e.g., RIP-seq).

Main Results:

  • High-throughput sequencing enables detailed analysis of lncRNA localization and function.
  • Specific methods like chromatin-associated RNA sequencing and nascent transcript Global run-on sequencing provide insights into lncRNA regulation.
  • Photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP) aids in identifying direct lncRNA-protein interactions.

Conclusions:

  • Current high-throughput sequencing approaches offer powerful tools for dissecting lncRNA biological functions.
  • These methods facilitate the understanding of lncRNA roles at promoter and enhancer elements.
  • Further application of these techniques will advance knowledge of lncRNA-mediated gene regulation.