Systemic morphofunctional assessment of ultrastructural organization and pathology of myocardial cells

A quantitative multifactorial method based on stereological parameters of ultrastructure has been worked out for unambiguous assessment of the condition of myocardial cells. The contraction force (F) generated by 1mm3 of myocardial cell mass, being the essential function of myocardial cells, had been chosen as a system-forming factor. Index F is calculated by our equation, as follows: F = K x Vvmf x (1/Svcr, 1/2.3Svmt, Vvspl/226.1, 1/2.3Svmf, Vv.mf/1533.7) x f x v1, where K describes the measure of dissocation of myofilaments and where the product of f x v1 is a constant. The data regarding the function of myocardial cell contractility measured by direct micromechanography were quantitatively parallel to those recordable by the new method, with one and the same animal being involved. Hence, evidence has been produced to the value of the new method in terms of objectivity and functional equivalence. This method can be used for quantitative testing for diagnosis and prognostication in theoretical and applied cardiology and in cardiac surgery.