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Updated: Mar 18, 2026

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
Published on: April 22, 2011
Nephronectin Expression Is Up-Regulated by BMP-2
Tamaki Kurosawa1, Atsushi Yamada, Dai Suzuki
1Department of Biochemistry, School of Dentistry, Showa University.
Abstract:
Nephronectin (Npnt), known to be a ligand of integrin α8β1, plays important roles in the development and function of various tissues, including those of the kidneys, liver, bones, and muscles. In previous studies, we showed that the expression of Npnt mRNA was regulated by various cytokines, including transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α), and oncostatin M (OSM), and that over-expression of Npnt enhanced osteoblast differentiation. In this study, we found that bone morphogenic protein-2 (BMP-2), known as an osteogenesis inducing cytokine, strongly up-regulated the expression of Npnt mRNA in a murine skeletal muscle cell line (C2C12) via the BMP-SMAD signaling pathway.
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