Simultaneous determination of diaveridine, trimethoprim and ormetoprim in feed using high performance liquid
Ya-Jun Yang1, Xi-Wang Liu1, Bing Li1
1Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China.
Abstract:
This study developed and validated a simple and reliable method for detecting and quantifying DVD, TMP and OMP in feed using dichloromethane extraction followed by HPLC-MS/MS. A matrix effect evaluation was performed using the post-extraction spiking method, and levels were less than ±15% in all three feeds with their corresponding concentrations. LOD and LOQ, CCα and CCβ were 20μgkg(-1) and 40μgkg(-1), 8.68-15.55μgkg(-1) and 10.61-18.92μgkg(-1) for all analytes, respectively. Calibration curves were linear for DVD, TMP and OMP with R(2)⩾0.990 and r⩾0.995, respectively. Recoveries of low, medium and high concentrations using the proposed method ranged from 74.4 to 105.2%. Repeatability and within-laboratory reproducibility were <7.4% (RSD). The chosen seven factors had no a significant influence on robustness. The method showed good performance when it was applied to analyze other laboratory-prepared or actual feed samples.
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