Localization, quantification, and characterization of pancreatic polypeptide immunoreactivity in the parasitic...

Pancreatic polypeptide (PP) immunoreactivity (IR) has been identified, quantified, and subsequently chemically characterised in the parasitic platyhelminth, Diclidophora merlangi, and its specific teleostean host the whiting, Merlangius merlangus. Immunocytochemistry demonstrated PP-IR throughout the central and peripheral nervous systems of the parasite and in open-type endocrine cells of the gastric mucosa of its host. Radioimmunoassay detected PP-IR in alcoholic extracts of whole parasites (39.2 ng/g) and in extracts of gastrointestinal tract (2.1 ng/g), brain (4.6 ng/g), and pancreas (12 ng/g) of the host. Chromatographic analysis of parasite extracts revealed a single immunoreactive species of PP in both high-performance gel permeation and reverse-phase systems. The molecular size of this peptide was similar to bovine PP standard. In contrast, whiting tissues contained two immunoreactive species of PP in both gel permeation and reverse-phase systems. The major species was similar in size to bovine PP standard and the minor species was smaller, with a molecular size comparable to bovine neurotensin. Reverse-phase HPLC revealed that parasite and host peptides were not identical.