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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Published on: January 17, 2015

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Combinatorial antibody libraries: new advances, new immunological insights

Richard A Lerner1

  • 1Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Nature Reviews. Immunology
|July 5, 2016
PubMed

Immunochemists have become quite proficient in engineering existing antibody molecules to control their pharmacological properties. However, in terms of generating new antibodies, the combinatorial antibody library has become a central feature of modern immunochemistry. These libraries are essentially an immune system in a test tube and enable the selection of antibodies without the constraints of whole animal or cell-based systems. This Review provides an overview of how antibody libraries are constructed and discusses what can be learnt from these synthetic systems. In particular, the Review focuses on new biological insights from antibody libraries - such as the concept of 'SOS antibodies' - and the growing use of intracellular antibodies to perturb cellular functions.

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