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Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

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Published on: June 23, 2011

Generating Cell Targeting Aptamers for Nanotheranostics Using Cell-SELEX.

Yifan Lyu¹, Guang Chen¹, Dihua Shangguan¹

¹1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.

|July 5, 2016

Summary

This summary is machine-generated.

This review details cell-based SELEX (Systematic Evolution of Ligands by EXponential enrichment) for creating aptamers. These aptamers show promise for disease diagnosis and cancer nanotheranostics.

Keywords:

Aptamers Cell-SELEX DNA network.aptamer drug conjugates nanotheranostic

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Area of Science:

Molecular Medicine
Biotechnology
Nanotechnology

Background:

Accurate disease diagnosis and therapy rely on understanding molecular-level cell changes.
Cell-based SELEX is a key technology for developing specific aptamers that target cells.
Aptamers are increasingly used in molecular medicine for biomarker discovery and cancer theranostics.

Purpose of the Study:

To review the cell-based SELEX process, including aptamer selection, identification, and characterization.
To discuss the potential of aptamers in constructing nanotheranostics for targeted therapy.
To explore the challenges and future prospects of cell-SELEX and aptamer-based nanotheranostics.

Main Methods:

Detailed mechanical description of the cell-SELEX process.
Discussion of aptamer selection, identification, and characterization techniques.
Review of aptamer applications in nanotheranostic construction.

Main Results:

Cell-based SELEX effectively generates highly specific, cell-targeting aptamers.
Aptamers show significant potential as targeting moieties in nanotheranostics.
The review covers current challenges and future directions in the field.

Conclusions:

Cell-based SELEX is a foundational technology for aptamer development in molecular medicine.
Aptamers are promising tools for biomarker discovery and targeted cancer therapy via nanotheranostics.
Further research into cell-SELEX and aptamer-based nanotheranostics is warranted to overcome existing challenges.