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Updated: Mar 18, 2026

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
Published on: April 25, 2013
Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale
Kevin C Zhou1, Brendan K Huang2, Ute A Gamm3
1Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, Connecticut 06520, USA; Current affiliation: Department of Biomedical Engineering, Duke University, Fitzpatrick CIEMAS, 101 Science Dr., NC 27708, USA; kevin.zhou@duke.edu.
Abstract:
We present a new OCT method for flow speed quantification and directional velocimetry: particle streak velocimetry-OCT (PSV-OCT). PSV-OCT generates two-dimensional, 2.5-vector component (vx ,|vy |,vz ) maps of microscale flow velocity fields. Knowledge of 2.5-vector components also enables the estimation of total flow speed. The enabling insight behind PSV-OCT is that tracer particles in sparsely-seeded fluid flow trace out streaks in (x,z,t)-space. The streak orientations in x-t and z-t yield vx and vz , respectively. The in-plane (x-z plane) residence time yields the out-of-plane speed |vy |. Vector component values are generated by fitting streaks to a model of image formation that incorporates equations of motion in 3D space. We demonstrate cross-sectional estimation of (vx ,|vy |,vz ) in two important animal models in ciliary biology: Xenopus embryos (tadpoles) and mouse trachea.
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