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Updated: Mar 18, 2026
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
SNP haplotyping technique for evaluation of MGP 5' UTR power in osteoblast cells
Abazar Roustazadeh1, Seyed Hamidreza Monavari2, Seyed Reza Hosseini Fard3
1Research Center for Non-Communicable Diseases and Biochemistry Department, Jahrom University of Medical Sciences, Jahrom, Iran.
Abstract:
Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells.
