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Updated: Mar 18, 2026
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C
Published on: April 29, 2020
UMI-4C for quantitative and targeted chromosomal contact profiling
Omer Schwartzman1,2,3,4, Zohar Mukamel3,4, Noa Oded-Elkayam3,4
1Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel.
Abstract:
We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.
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